Projekt

As macroscopic systems, cells exhibit biochemical properties that are heavily influenced by the surrounding extracellular matrix. The project aims at rewiring living cells by in-situ lateral organization of membrane receptors and thus exert control over signaling networks by light. Wavelength-selective as well as two-photon photoactivatable supramolecular interaction pairs will be developed to direct receptor clustering with high spatiotemporal resolution. The one- and two-photon activation will facilitate a non-invasive protein organization in 2D and 3D. Combined with superresolution microscopy and single-molecule imaging, the multi-photon patterning will provide valuable insights into suprastructures of receptors and their impact on cell response. The approach will ultimately allow to control receptor clustering in a macroscopic 3D cell culture at system-level.

• Prof. Dr. Robert Tampé

  Universität Frankfurt am Main
  Fachbereich Biochemie, Chemie und Pharmazie
  Institut für Biochemie, Biozentrum
  Zelluläre Biochemie
  N210
  Frankfurt am Main
  

• Dr. Ralph Wieneke

  Universität Frankfurt am Main
  Fachbereich Biochemie, Chemie und Pharmazie
  Institut für Biochemie
  Zelluläre Biochemie
  Biozentrum, N210
  Frankfurt am Main
  

• Prof. Dr. Mike Heilemann

  Universität Frankfurt am Main
  Fachbereich Biochemie, Chemie und Pharmazie
  Institut für Physikalische und
  Theoretische Chemie
  Single Molecule Biophysics
  Frankfurt am Main