Daten zum Projekt

Design of [FeS]-cluster containing Metallo-DNAzyme subunits for modular redox-chemistry applications

Zur Projekt-Website

Initiative: Integration molekularer Komponenten in funktionale makroskopische Systeme (beendet, nur noch Fortsetzungsanträge)
Bewilligung: 26.06.2017
Laufzeit: 3 Jahre


Iron sulfur [FeS] clusters are highly efficient redox centers that are widely and effectively used by natural metalloproteins in biological redox-reactions. Due to their electrochemical properties, including a finely tunable redox-potential, [FeS]-clusters have a great potential for applied science, including the constitution of defined electron transfer pathways and catalytic redox centers. However, [FeS]-clusters have not been successfully reconstituted within biomolecular building blocks of bio-derived or semi-synthetic materials such as aptazymes or DNA origami-elements. The collaboration between the Happe and the Willner group unites the required expertise in the complementary fields of metalloprotein-research and nucleoapzyme design to tackle this challenging task. They will follow three approaches to realize [4Fe4S]-cluster reconstitution in different types of DNA-scaffolds (aptamer-based, G-quadruplex-based and rigid polygonal DNA-building blocks). After thorough spectroscopic verification of cluster synthesis they will reconstitute two types of catalytic redox systems, the electron relay of sulfite-/nitrite-reductase and the H2-producing H-cluster of [FeFe]-hydrogenases.


  • Prof. Dr. Thomas Happe

    Universität Bochum
    Fakultät für Biologie und Biotechnologie
    Lehrstuhl Biochemie der Pflanzen
    AG Photobiotechnologie

  • Prof. Itamar Willner, Ph.D.

    The Hebrew University of Jerusalem
    Institute of Chemistry
    Department of Organic Chemistry

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