Projekt

Daten zum Projekt

Assembly of supramolecular frameworks for 3D bioprinting (extension)

Initiative: Integration molekularer Komponenten in funktionale makroskopische Systeme (beendet, nur noch Fortsetzungsanträge)
Bewilligung: 24.06.2019
Laufzeit: 3 Jahre

Projektinformationen

Cells, as macroscopic systems, exhibit biochemical properties that are strongly influenced by the surrounding extracellular matrix. The aim of the project is to rewire living cells by in situ lateral organization of membrane receptors and thus exert control over signaling networks by light. Wavelength-selective as well as two-photon photo-activatable supramolecular interaction pairs will be developed to direct receptor clustering with high spatiotemporal resolution. The one- and two-photon activation will facilitate a non-invasive protein organization in 2D and 3D. Combined with super-resolution microscopy and single-molecule imaging, the multi-photon patterning will provide valuable insights into suprastructures of receptors and their impact on cell response. The approach will ultimately allow control of receptor organization in a macroscopic 3D cell culture at the systemic level.

Projektbeteiligte

  • Prof. Dr. Robert Tampé

    Universität Frankfurt am Main
    Fachbereich Biochemie, Chemie und Pharmazie
    Institut für Biochemie, Biozentrum
    Zelluläre Biochemie
    N210
    Frankfurt am Main

  • Prof. Dr. Mike Heilemann

    Universität Frankfurt am Main
    Fachbereich Biochemie, Chemie und Pharmazie
    Institut für Physikalische und
    Theoretische Chemie
    Single Molecule Biophysics
    Frankfurt am Main

  • Dr. Ralph Wieneke

    Universität Frankfurt am Main
    Fachbereich Biochemie, Chemie und Pharmazie
    Institut für Biochemie
    Zelluläre Biochemie
    Biozentrum, N210
    Frankfurt am Main