Daten zum Projekt
Initiative: | Integration molekularer Komponenten in funktionale makroskopische Systeme (beendet, nur noch Fortsetzungsanträge) |
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Bewilligung: | 24.06.2019 |
Laufzeit: | 3 Jahre |
Projektinformationen
Cells, as macroscopic systems, exhibit biochemical properties that are strongly influenced by the surrounding extracellular matrix. The aim of the project is to rewire living cells by in situ lateral organization of membrane receptors and thus exert control over signaling networks by light. Wavelength-selective as well as two-photon photo-activatable supramolecular interaction pairs will be developed to direct receptor clustering with high spatiotemporal resolution. The one- and two-photon activation will facilitate a non-invasive protein organization in 2D and 3D. Combined with super-resolution microscopy and single-molecule imaging, the multi-photon patterning will provide valuable insights into suprastructures of receptors and their impact on cell response. The approach will ultimately allow control of receptor organization in a macroscopic 3D cell culture at the systemic level.
Projektbeteiligte
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Prof. Dr. Robert Tampé
Universität Frankfurt am Main
Fachbereich Biochemie, Chemie und Pharmazie
Institut für Biochemie, Biozentrum
Zelluläre Biochemie
N210
Frankfurt am Main
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Prof. Dr. Mike Heilemann
Universität Frankfurt am Main
Fachbereich Biochemie, Chemie und Pharmazie
Institut für Physikalische und
Theoretische Chemie
Single Molecule Biophysics
Frankfurt am Main
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Dr. Ralph Wieneke
Universität Frankfurt am Main
Fachbereich Biochemie, Chemie und Pharmazie
Institut für Biochemie
Zelluläre Biochemie
Biozentrum, N210
Frankfurt am Main